GEM-IL: A highly responsive fluorescent lactate indicator
Published on 10.21.2021 in Cell Reports Methods
Ramsey Bekdash, Jose R. Quejada, Shunnosuke Ueno, Fuun Kawano, Kumi Morikawa, Alison D. Klein, Kenji Matsumoto, Tetz C. Lee, Koki Nakanishi, Amy Chalan, Teresa M. Lee, Rui Liu, Shunichi Homma, Chyuan-Sheng Lin, Maria V. Yelshanskaya, Alexander I. Sobolevsky, Keisuke Goda, Masayuki Yazawa
The ability to detect and monitor changes in lactate at the cellular level is essential for understanding various physiological systems. Current methods have limited sensitivity and low intracellular expression, and there are no genetically encoded in vivo models available. To address these issues and monitor changes in lactate, we have developed, optimized, and characterized a genetically encoded metabolic indicator for lactate (GEM-IL). This tool is a reliable method for studying metabolic activity as demonstrated in vitro and in situ. In addition, we have developed an in vivo murine model for this indicator for further applications in metabolism study.
•Development of a genetically encoded metabolic indicator for lactate (GEM-IL)
•Mutagenesis and fluorophore alteration improve responsivity and specificity to lactate
•In vivo model generation, validation, and application of GEM-IL
•GEM-IL shows improved sensitivity over currently available lactate indicators