Expression, purification, and crystallization of full length ionotropic glutamate receptors
Published on 01.07.2016 in Neuromethods
Maria V. Yelshanskaya, Kei Saotome, Minfen Li, Alexander I. Sobolevsky
Ionotropic glutamate receptors (iGluRs) are ligand-gated ion channels that mediate the majority of excitatory neurotransmission in brain and are implicated in nearly all aspects of central nervous system development and function. There is great clinical interest in iGluRs because their dysfunction is associated with chronic neurodegenerative conditions, psychiatric disorders, brain trauma and stroke. Despite physiological and pathophysiological importance, obtaining structural information about full-length iGluRs has proven to be a difficult task. To facilitate further structural/functional studies, we describe a detailed protocol of the full-length iGluR expression, purification, and crystallization.